Background: In the recent years, vitamin D has become a topical subject and a focus of research not only in reproductive medicine but across many medical disciplines. In reproductive medicine, studies have identified an association between vitamin D status in women and ovarian reserve, Aim and objectives: the aim of the study was to detect the relationship between vit D and low antral follicle count in infertile females below 35 years old, Subjects and methods: This was a cross-sectional observational study that was conducted at the outpatient clinic of obstetrics and gynecology in Benha university hospital and Tokh central hospital, The study was carried out on  50 infertile patients below 35 years old with low AFC diagnosed first by ultrasonography then vit d level was done for those patients to detect the relation between vit d and low AFC, Results: Patients' ages ranged from 22 to 34 years with mean of 25.98 years and median was 25 years. Mean ± SD of AFC among participant women was 4.35 ± 1.41, ranged from 4 to 6 years and median was 4 . Mean ± SD of Vitamin D was 18.67 ± 14.58(ng/ml) ranged from 13.5  to 28.2 (ng/ml)  and median was 19.1 (ng/ml). There is a non-significant correlation between vitamin D and (Age, AFC and BMI).Conclusion: After conducting this study we could not found a significant correlation between vit D level and low antral follicle count. Future studies are required to investigate further interaction between vit D level and infertility.