Introduction. Pomegranate is an old fruit which has many antimicrobial compounds. Impact of pomegranate peel extracts on Escherichia coli clinical isolate from Benha general hospital was investigated in this study. Material and Methods: pomegranate peel extraction was primed and GC/MS chromatogram analysis was carried out using the GC Agilent Technologies gas chromatography fitted with HP-5 fused silica column (25mm × 0.25mm, film thickness of 0.25 µm) and interfaced to the flame-ionization detector (FID). The antibacterial potential of Pomegranate peel extractions against E.coli sample was done using well diffusion method. Results: According to (GC/MS) technique, methanol and aqueous extracts of pomegranate peel have inhibitory effects on E. coli isolate.  The minimum inhibition concentrations (MICs) of methanolic and aqueous extracts were 100 and 60 mg/L, respectively. In this study , the MICs values of Aqueous extract of pomegranate peel  were higher than of methanolic extract of pomegranate peel (P<0.05).  Zones of inhibition and  values of MICs indicated that the  aqueous extract of pomegranate peel  had more effect on  E. coli isolate than  methanolic extract of pomegranate peel. Conclusion: In this study, the extraction of pomegranate peel has antibacterial effect on  E.coli isolate. However, the aqueous extract had a higher effect on E.coli than the methanolic extract.