Acne vulgaris is a common skin condition that affects people all over the world. Acne vulgaris is characterised by the presence of open and closed comedones, papules, pustules, and nodules on areas of the body with hormonally sensitive sebaceous glands. Acne is thought to be caused by four key factors: increased sebum production, follicular hyperkeratinization, skin bacterial colonisation, and inflammation. Acne with severe symptoms is painful, disfiguring, scarring, and lowering self-esteem. The aim of this study was to determine the serum level of calprotectin in acne vulgaris patients and its relationship to disease severity. Subjects and Methods: In this sample, 60 people with acne vulgaris were paired with 30 people who appeared to be of similar sex and age as a control group. The patients were chosen from those who attended the Benha University Hospital's Dermatology and Andrology Department's Outpatient Clinic between April 2019 and October 2020. Our findings indicated that the AV group had a statistically significant higher level of serum calprotectin with mean SD (6.291.91ng/dl) than the control group with mean SD (2.040.65ng/dl) (p=0.004). The amount of serum calprotectin had a statistically significant positive relationship with the GAGS score (r=0.854; p0.001). The period of the disease, however, had no meaningful correlation (r=0.163;p=0.212). Conclusion: Serum calprotectin levels are higher in acne vulgaris patients relative to safe controls, and there is a statistically significant relationship between calprotectin levels and disease severity, suggesting that calprotectin may be used as a chemical biomarker to determine disease severity.