Breast cancer (BC) is the most common type of cancer encountered in women and the second leading cause of death after lung cancer. Thus, BC is a serious health concern for all women. The long noncoding RNA (lncRNA) H19 is a maternally expressed imprinted gene that plays important roles in tumorigenesis, progression, and metastasis. lncRNA H19 has been well studied playing an important role in BC progress and the expression of H19 may service as a diagnostic target for BC. Polymorphisms in lncRNA H19 have been shown to be associated with the risk of cancer, but the findings are inconsistent. The present study aimed to investigate the effect of lncRNA H19 single nucleotide polymorphism (SNP) rs217727 in Egyptian breast cancer females. We determined the genotypic frequency of lncRNA H19 rs217727 in 100 breast cancer patients, 50 women with benign lesion and 50 healthy individuals using PCR and gel electrophoresis.We found significant association between lncRNA H19 rs217727 polymorphism and BC as the T allele was significantly higher in BC patients than benign and control groups.It is indicated in this research that rs217727 is statistically correlated with the Bsusceptibility of BC in Egyptian females.