Nanoparticles plans to overcome the problems related to human diseases at the nanoscale level and making significant contributions to the improvement of new approaches of drug delivery in cancer and can provide a stage for combined therapeutics with subsequent monitoring of response. (Garlic and zinc oxide (ZnO) coated by cetyl trimethyl ammonium bromide) nanoparticles have been applying treatment action against hepatocellular carcinoma in vitro and in vivo which was done on 60 rats and was divided into four groups, each groups have (15 rats), (Group 1) NTBR (Negative control) group; (Group 2) (TBR) HCC (DEN) group (Group 3) (TBRT) (garlic / Zinc oxide / CTAB) nanoparticles; (Group 4) (TBRT Doxorubicin) .The changes of the biochemical indices of serum, and the levels of the Cell cycle, Caspase 3and DNA fragmentation percent of liver were analyzed to evaluate the treatment activity. At the end of the study (10 weeks), DEN induced elevation of the serum biochemical indices alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, gammaglutamyl transferase, Alpha feto-protein, (garlic/Zinc oxide/ CTAB) nanoparticles administration significantly inhibited the increase of the ALT, AST, ALP, GGT and AFP .The mechanistic studies demonstrated that (garlic/Zinc oxide/CTAB) nanoparticles counteracted DEN induced protein levels of Caspase 3and DNA fragmentation percent were significantly increased. We observed that nanocompsite have different effects on liver cell viability via killing cancer cells, while posing no effect on normal cells of the liver. The marked difference in cytotoxicity between cancer cells and normal cells suggests an exciting potential for nanocompsite as novel alternatives to hepatocellular carcinoma therapy