The aim of this study was updating the information about the prevalence of infestation with Rhinoestrus spp. larvae in Egypt and as a result determining the correct time for chemotherapy. 303 Egyptian donkeys (Equus asinus) of different ages and sex slaughtered at Giza Zoo slaughter house from June 2014 to May 2015 were examined for the presence of Rhinoestrus spp. larvae. A total of 8388 Rhinoestrus spp. larvae [7221 L1 (86.08%), 545 L2 (6.49%), 622 L3 (7.41%)] were recovered from 74.91% of donkeys with an overall mean of 27.68 ± 2.43 larvae per head. Heavier infestations were recorded in winter and summer. There were more than two generations of Rhinoestrus spp. occurred in the year. Two yearly treatments during June and November are recommended for eradication of such infestation from donkeys.