Bone morphogenetic proteins (BMPs) are growth factors belonging to the transforming growth factor beta (TGF-β) superfamily. BMPs are intra-ovarian factors expressed in mammalian ovaries by oocytes, granulosa and theca cells. The present study was designed to isolate and detect polymorphism in BMP 4 (exon 2 plus part of intron 2) gene associated with infertility in Egyptian buffalo cows using PCR-RFLP and nucleotide sequencing techniques. PCR amplification of DNA from 35 Egyptian water buffalo (5 normal fertile, 14 anestrum and 16 repeat breeders) with 482 bp expected size was purified and sequenced. There was no variation in the sequence of amplified region between fertile and infertile group. PCR product was digested using Taqα1 and Hinf1 restriction enzymes gave one type of the restriction banding pattern with no polymorphism. Concluded that BMP 4 is highly evolutionarily conserved gene.