Hypercholesterolaemia disorder is characterized by very high level of cholesterol in the blood which is cardiovascular health risk. Chemical compound Medicines have serious side effects that cause an imbalance in the body's functions. Therefore, utilization of natural compounds could be an alternative concept in the treatment of diseases, as they have no side effects on human health. The present work aims to evaluate the physiological effects of royal jelly (RJ) on the hypercholestrolaemic male albino rats. Forty-two male albino rats were divided into six groups. Rats were treated with coconut oil, cholesterol (450 mg/kg b.w of cholesterol dissolved in 0.5 ml coconut oil) for four weeks, RJ treated group (300 mg/kg b.wt) for two weeks, RJ before and after cholesterol treated groups. Serum cholesterol showed significant increased levels of triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C) and very low-density lipoprotein cholesterol (VLDL-C), and significant decrease in high density lipoprotein cholesterol (HDL-C), antioxidant enzymes, nitric oxide (NO) and vascular endothelium growth factor (VEGF) compared with those of control and other treated groups. Royal jelly administration reduced the high levels of LDL-C, VLDL-C, TG and TC because of cholesterolaemia. Also, RJ could improve the levels of HDL-C, serum antioxidant enzymes, NO and VEGF. Treatment with RJ before and after cholesterol could help to return serum NO, VEGF levels toward control values and causes significant decrease in LDL-C level and significant increase in HDL-C level compared to control group. In conclusion, RJ ameliorates the harmful effects of cholesterol administration on male albino rats and plays an important role in cardiovascular health protection