Two field experiments were carried out during the two successive seasons of 2014/2015 and 2015/2016 in private sector farm at El–Dair village, Kalubia governorate in sandy soil to investigate the response of two strawberry cultivars namely Fortuna and Sweet Charlie, transplants root dipping or without dipping in methylotrophic bacteria and sprayed up to six times with methylotrophic bacteria (10 cm3 /l) on vegetative growth, chemical composition and productivity of some strawberry cultivars. Obtained results showed that there were significant differences among the studied strawberry cultivars in all measured vegetative growth traits, fruit yield and its components as well as fruit quality. In this respect, cv. Fortuna reflected the highest values of vegetative growth, chemical composition of plant foliage, fruit yield and its components and physical and chemical fruit quality. Also foliar spraying plants six times with methylotrophic bacteria at 10 cm3 /l starting after 20 days from transplanting and every 15 days by intervals during the growth season was superior in total and marketable fruit yield. Different tested bio-fertilization (methylotrophic bacteria) enhanced the vegetative growth, chemical constituents of plant foliage, total produced fruit yield and its components as well as fruit quality. In addition, using methylotrophic bacteria at 10 cm3 /l reflected the highest values in all studied growth and yield traits of tested cultivars.