Volatile oils obtained from Rosmarinus officinalis plants treated with recommended doses of organic and
mineral fertilizers (VO1); plants treated with organic, mineral and bio fertilizers (VO2); plants treated with
combination of organic, mineral, bio fertilizers and Biomagic (VO3) were analyzed by Gas chromatography
(GC), it was illustrated that VO3 was better than VO1 and VO2 concerning known chemical constituents. Moreover,
VO1, VO2 and VO3 were screened for their antibacterial, antioxidant, antitumor and antidiabetic activities. VO3 were more
effective in inhibiting the growth of tested bacteria whereas VO1 was the least inhibitive. On the other hand the antitumor
results revealed that VO3 exhibited the lowest inhibitory concentration 50 (IC 50) against MCF-7 carcinoma cell line in
comparison to the other oils. The three oils were analyzed for their antioxidant activities using 2,2-Diphenyl-1-Picrylhydrazyl
(DPPH%) scavenging activity method. VO3 revealed the highest DPPH scavenging % in comparison to the other oils. The
results showed that alpha glucosidase diabetes enzyme was inhibited by all the tested oils in dose dependent manner but with
superiority to VO3. This study showed great prospects in effectiveness of biofertilizers in combination with Biomagic on
constituents of R. officinalis oil and subsequently on its biological activities. Thus, this study points to investigate the
combination of biofertilizers and Biomagic as a potential tool to improve biological activities of plant volatile oils.