Previously, the only way to get reliable information about the stomach mucosa had been gastroscopy and histological analysis of the biopsy. Many studies have been reported on the evaluation of blood tests such as gastropanel (pepsinogen I, II and gastrin 17) to predict normal gastric mucosa and screening markers for chronic atrophic gastritis.was to compare endoscopic gastric biopsy with serum pepsinogen II (PG II) and serum gastrin 17 (G-17) to detect their association with H. pylori gastritis. This study included 75 patient more than 18 years old with recurrent abdominal pain that motive the performance of Upper Gastrointestinal Endoscopy and 90 apparently healthy individual matched for age and sex. Serum pepsinogen II and serum gastrin 17 were quantitated using Enzyme Linked ImmunoSorbent Assay (ELISA).Upper GIT endoscopy and histopathology of gastric and duodenal biopsies obtained from 75 consecutive patients undergoing upper gastrointestinal endoscopy for the investigation of recurrent abdominal pain were done.Our study histopathology of gastric ,doudenal biopsies showed that 32/75 (42.7) of H.pylori positive patients have H.pylori-associated gastritis and duodenitis with mild activity & mild H.pylori density (+).Serum Pepsinogen II ( ng/ml)Mean ± SD of H.pylori +ve group (24.54 ± 8.66 ) was higher than H.pylori -ve group which was(4.55 ± 2.37)with p value (<0.001). serum gastrin 17 ( pg/ml)Mean ± SD of H.pylori +ve group was (240.68 ± 231.77) was higher than H.pylori -ve group (29.26 ± 5.41) with p value (<0.001).Serum PG II and gastrin 17 can be a useful non-invasive marker for H.pylori gastritisn with recurrent abdominal pain with no apparent organic reason.