In Threatened PTL patients, due to inflammation and possible ongoing ischemia process, platelet activation indices like MPV and PDW should be altered than the controls.The aim of this study was to evaluate the alterations of platelet indices and hemoglobin level and to investigate whether they can be used as a predictor marker for threatened preterm birth or not.This study was a case control study included 60 pregnant women attending to the department which was divided into two groups: Study group (30 women): with threatened preterm labor and Control group (30 women): healthy pregnant wome Hemoglobin levels were measured and Platelet indices: mean platelet volume (MPV); platelet distribution width (PDW). Lower hemoglobin levels in TPL group. Also, MPV was lower in TPL group while PDW was higher in PTL group. hemoglobin level less than 9.07 g/dl may predict TPL also, MPV levels may be used for discriminating high risk population for preterm labor. By using a cut-off value 8.59 for MPV with sensitivity and specificity of 64%, 46% respectively PDW in this study revealed an area under curve (AUC) 63%. By using a cut-off value 15.98 for PDW, sensitivity was 68% and specificity was 47% for TPL. .Conclusion: Preliminary findings of this study showed that hemoglobin level less than 9.07 g/dl may predict TPL also, MPV levels may be used for discriminating high risk population for preterm labor. Further prospective studies with larger sample size are needed to confirm this finding