Foundation: Alopecia areata is a non-scarring going bald condition that can happen and repeat in male or female at any age and on any piece of hair-excepting regions. Zinc has a significant part in different metabolic pathways and cell capacities. Biotin engaged with fundamental physiological capacities like unsaturated fat combination, amino corrosive catabolism, and gluconeogenesis. The point of the current examination was to assess serum zinc and biotin level in patients with AA. Subjects and strategies: This investigation was led on sixty patients experiencing AA. Notwithstanding sixty clearly solid people of coordinated age, sex and BMI as a benchmark group. Serum level of zinc and biotin were estimated in the two gatherings. Results; Results of the current investigation demonstrated huge contrasts with respect to serum zinc and biotin levels in AA patients when contrasted with control gathering. there was a noteworthy relationship between's serum zinc and serum biotin.Conclusion: zinc and biotin serum levels were lower in AA patients contrasted with controls, which may assume significant function in the pathogenesis of the sickness. What's more, this examination may give significant insights to help the improvement of new restorative systems for patients with AA.