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135449

Evaluation of Nano-Based-ELISA for Serodiagnosis of Human Toxoplasmosis

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Last updated: 28 Dec 2024

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Abstract

Toxoplasma surface antigen 1(SAG1) is a major surface antigen involved in the diagnosis of toxoplasmosis. Although it only accounts for 3-5% of total T. gondii protein, the majority of the antibodies are reactive against SAG1 during infection. SAG1 has potent immunogenicity and immune-reactivity; therefore, it is widely applied in diagnostic techniques. Will assess the symptomatic execution of a novel nano symptomatic elisa test Previously, serodiagnosis for human toxoplasmosis. Generation for list 1 What's more its purification might have been carried accompanied Eventually Tom's perusing preparation about igigi polyclonal antibodies Also its purification, characterization Furthermore conjugation with gold nanoparticles identification of Toxoplasma (SAG1) in the serum by nano-gold sandwich elisa uncovered 89. 2% affectability Also 94%, specificity. Nano-gold elisa method is a delicate test to identification of mankind's toxoplasmosis.

DOI

10.21608/bjas.2020.135449

Authors

First Name

A.S.

Last Name

Al hamshary

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-

Affiliation

Parasitology Dept., Faculty of Medicine, Benha Univ., Benha, Egypt

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First Name

I.R.

Last Name

Bayoumi

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Affiliation

ImmunoParasitology Dept., Theodor Bilharz Research institute, Imbaba, Giza, Egypt

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First Name

N.S.

Last Name

Aly

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-

Affiliation

Parasitology Dept., Faculty of Medicine, Benha Univ., Benha, Egypt

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First Name

R.E.

Last Name

Omar

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-

Affiliation

Parasitology Dept., Faculty of Medicine, Benha Univ., Benha, Egypt

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First Name

D.A.

Last Name

Mohammed

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-

Affiliation

Parasitology Dept., Faculty of Medicine, Benha Univ., Benha, Egypt

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First Name

Y.M.

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Marei

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-

Affiliation

Biochemistry and Molecular Biology,Dept., Faculty of Medicine, Benha Univ., Benha, Egypt

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First Name

G. A.

Last Name

Rashed

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-

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Parasitology Dept., Faculty of Medicine, Benha Univ., Benha, Egypt

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Volume

5

Article Issue

Issue 2 part (1)

Related Issue

20198

Issue Date

2020-02-01

Receive Date

2020-01-04

Publish Date

2020-02-01

Page Start

161

Page End

167

Print ISSN

2356-9751

Online ISSN

2356-976X

Article File

BJAS_Volume 5_Issue Issue 2 part (1)_Pages 161-167.pdf

PDF . 897.2kB

Link

https://bjas.journals.ekb.eg/article_135449.html

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https://bjas.journals.ekb.eg/service?article_code=135449

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24

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Original Research Papers

Type Code

1,647

Publication Type

Journal

Publication Title

Benha Journal of Applied Sciences

Publication Link

https://bjas.journals.ekb.eg/

MainTitle

Evaluation of Nano-Based-ELISA for Serodiagnosis of Human Toxoplasmosis

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Article

Created At

23 Jan 2023