Introduction: Orthodontically induced inflammatory root resorption is the most unpredictable side effects of orthodontic treatment. The objective of this prospective randomized split mouth trial was to investigate the effect of low level laser therapy on orthodontically induced root resorption using cone beam computed tomography. Methods: The sample consisted of 10 patients (18-24 years old) with the therapeutic need to extract the maxillary first premolars and subsequent canine retraction. All patients maxillary quadrants were randomly allocated to either the experimental side (receive a low level diode laser with a wavelength of 910 nm) or the control side (receive a placebo laser). The low level laser was applied days 0, 1, 2, and 3 of canine retraction and then weekly during the 3 month of the study period. Bilaterally, canine retraction was performed with closed-coil nickel-titanium springs that applied 150g of force on each side. The root length of the maxillary canine and maxillary first molar on both sides were compared and length reduction was measured from the pre- and post-treatment cone beam computed tomography scans to quantify OIRR. Results: There was a statistically significant decrease in mean root length post-treatment for both experimental and control sides. However, the mean amounts of root resorption at the experimental and control sides was not statistically significant. Conclusions: The incidence of orthodontically induced inflammatory root resorption on the Low level laser therapy side was lower than in the control group, yet, statistically insignificant.