T his study reported on a sporadic, naturally acquired infection of sheep and goats with Brucella on a private farm in El- Menofya Governorate, Egypt. The abortions, which occurred in a flock of 46 sheep and 33 goats, involved six ewes and five does at the third month of gestation. Serum samples from the flock were examined for Brucella antibodies using rose bengal test (RBT), Tube Agglutination Test (TAT) , indirect Enzyme Linked Immunosorbent Assay (I ELISA) and were further confirmed using complement fixation test (CFT) gave 18(39.1%), 16(34.8%), 19 (41.3%), and 17(37%) in sheep; respectively, while in goats revealed 11 (33.3%), 8(24.2%), 12(36.4%), and 11(33.3%) respectively. Tissue samples were collected from 28 positive animals, as detected by CFT, at slaughtering. The bacteriological results revealed 44 isolates which were biochemically identified as B. melitensis biovar3. The highest recovery rate was obtained from supra mammary lymph nodes (22/28; 78.6%), followed by spleen (15/28; 53.6%), and finally liver (7/28; 25%).The distribution of the virulence genes among 44 B. melitensis isolates revealed that Omp 25 recorded the highest incidence 44(100%), then followed wbkA 43(97.7%) and manB 42 (95.5%) . The high prevalence of virulence-associated genes among the B. melitensis isolates detected from different animal species in Egypt indicates a potential virulence of this bacterium. The authors concluded that the most frequent virulence genes are wbkA, manB and omp25 among isolates which are assumed to play a worthy function in the pathogenesis of brucellosis in this region