Antimicrobial resistance is considered as one of the most important issue that has a negative impact on poultry industry worldwide as well as of public health concerns. In this study, antimicrobial resistance pattern of Salmonella species and Escherichia coli, isolated from broiler chickens and migratory birds in Egypt, was investigated. A total of 250 diseased broiler chickens and 100 migratory birds (five bird species) were collected. All collected samples were then subjected to Salmonella and E. coli isolation, identification and antimicrobial susceptibility pattern. Salmonella and E. coli were isolated from broiler chickens with an incidence of (14%) and (40%) respectively, and from migratory birds with an incidence of (10%) and (30%) respectively. Based on serotyping, three serotypes were detected from broiler chickens (S. typhimurium, S.kentucky and S. enteritidis) and four Salmonella serotypes from migratory birds (S. typhimurium, S. kentucky, S. infantis and S. enteritidis). Twelve E. coli serotypes found in samples collected from broiler chickens (O1, O6, O55, O86, O91, O124, O125, O128, O144, O158, O159 and O166) while E. coli serotypes obtained from migratory birds, were found as: O1, O2, O26, O55, O78, O91, O113, O121, O124, O 128, O146 and O158.Overall,the antimicrobial susceptibility testing of isolated Salmonella and E. coli from broiler chickens and migratory birds showed resistance to most of the used antimicrobial agents; Salmonella isolates obtained from broiler chicken farms were found totally resistant to amoxicillin, ampicillin-sulbactam, and tetracycline. E. coli isolates obtained from broiler chicken farms showed resistance ranged from 25% - 95%. Salmonella and E. coli isolates retrieved from migratory birds showed resistance which ranged from 60% - 100% and 66.7 % - 96.7% respectively. This study highlights the spread of antimicrobial resistance Salmonella and E. coli in both domestic chicken and migratory birds. Hence, strict biosecurity measurements and continuous monitoring of antimicrobial resistance among poultry population should be implemented in addition to avoid the misuse of antimicrobial agents.