Trace element deficiency in male lambs is a serious problem. It refers to either insufficient nutrients or mineral elements imbalance. This work aimed to investigate the hormonal and some biochemical changes related to some trace element deficiency and the effect of mineral mixture supplementation on these changes. To achieve this aim we induced trace element deficiency in a tested male lambs group (N=10) belonged to our sheep herd and received a mineral mixture as a feed supplement for 10 days. The analysis of tested diet revealed protein 12.32%, calcium 1.21%, phosphorus 0.27%, and zinc 25.96 mg/kg DM. While the control healthy group (N=10) received a traditional diet. The results of serum hormones and biochemical analysis of tested group revealed a significant (P˂0.05) decrease in TSH, T3, T3/T4, testosterone, free testosterone, SHBG, GH, albumin and zinc, with a significant (P˂0.05) increase in T4, total protein and globulin compared to control healthy group. On the other hand, the results obtained after mineral mixture supplementation revealed a significant (P˂0.05) increase of TSH, T3, T3/T4, testosterone, free testosterone, SHBG, GH, albumin and zinc. Also, a significant (P˂0.05) decrease in T4, total protein, and globulin was observed compared with this group before mineral mixture supplementation, with observation of the changes were gradually toward recovery. Moreover, insignificant changes were recorded in calcium, phosphorus and iodine. In conclusion to trace element deficiency have an effect on vital hormones with extension to metabolic process, general health condition and proper growth of male lambs.