A total of  150  samples were collected from 110 diseased and 40 dead rabbits from private farms including (50 abscess swabs,20 liver samples,20 lung samples,30 infected wound swabs and 30 mastitic milk samples ) for bacteriological and molecular examinations.  S. aureus was isolated from 27 cases with a percentage of 18 %, including 7 isolates resistant to methicillin (MRSA) with a percentage of 4.7 % and 20 isolates were sensitive to methicillin with a percentage of 13.3%.
Sixty rabbits were used as laboratory animal models in an experiment designed to study the pathogenicity of S. aureus. rabbits were monitored daily for development of clinical signs in the form of abscess formation, conjunctivitis, keratitis, arthritis, cough, weight loss or lethargy.
MRSA experimentally injected strain could be recovered and confirmed bacteriologically and molecularly form all infected group rabbits.
All S. aureus strains were positive for amplification of 756 base fragments specific for 16S rRNA of S. aureus using Staph756 F and Staph750 R primers, while 7 MRSA strains showed positive amplification of 450 base pair fragments specific for SCCmec subtype IVa genes using SCCmec 4a1 and SCCmec 4a2 primers.
 Four  Rep-PCR primers were used for estimating of genetic diversity of MRSA isolates ( Rep-1, Rep-2, Rep-12and Rep-18 ). The four Rep-PCR primers produced about 55 fragments, 26 of them consider as monomorphic bands with about 47.5% and other 29 fragments consider as polymorphic bands with about 52.5%.