Lumpy skin disease virus (LSDV) was detected in skin biopsies which were collected from diseased animals surfing from LSD. in Eldakhla El-Wady El-Gedid Governorate in 2020.The virus was identified by electron microscopic (EM) examination and confirmed by molecular characterization through sequence and phylogenetic analysis. A phylogenetic analysis was performed using partial sequencing of the ORF103 gene and comparing with reference LSD viruses' isolates obtained from Gene Bank. The results of the sequence analysis were similar among themselves (99.4-100% identity), it was shown 100% nucleotide genetic similarity with Egyptian isolates of LSDV(CPD/Menofiya 1/2018 MK 342935) and LSD isolate (El Wadi El-Gedid 2018 MN792930)and identities were (99.8% ) with LSD (Serbia/Bujanovac/2016/KY702007).and LSD (Russia/Dagestan/2015MH893760.) where identities were (99.4%) with LSD (Kenya/MN072619) and LSD (KZ- Kostanay MT992618) .and they were  found different from  the other LSD viruses around the world. Further investigation, ELISA test was applied and the specific antibodies of LSDV were detected in serum samples of diseased animals (93.33%) and some of contact animals (10% ).