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184692

Use of ELISA and polymerase chain reaction as rapid different methods in diagnosis of enterotoxemia in foals

Article

Last updated: 04 Jan 2025

Subjects

-

Tags

Animal Health

Abstract

Necrotizing enterocolitis is a serious disease of sudden onset in foals associated with a high death rate, despite therapeutic intervention. Clinical manifestations of enterocolitis in foals vary from being found dead to spectrum clinical manifestations.
In this study, 50 fecal samples collected from foals aged 1-4 months and suspected clinically to be suffered from enterocolitis with main symptoms of profuse diarrhea, colic and abdominal pain. Rapid diagnosis was essential for controlling the disease instead of animal inoculation which is morally forbidden. Two rapid methods were used for diagnosis; ELISA and PCR tests. Out of 50 samples, 22 Clostridium perfringens isolates were detected (44%) and typed according to their major toxins and the presence of toxin genes using ELISA and PCR respectively. By PCR, 18 isolates were found to harbor cpa gene only (type A) and 4 isolates harbored cpa and cpb gene (type C), however, ELISA revealed that only 12 isolates have toxigenic activities for alpha toxin (type A), 6 isolates were non-toxigenic and 4 isolates gave alpha and beta toxins (type C) meaning that 6 isolates were not expressed by conventional PCR .

DOI

10.21608/ejah.2021.184692

Keywords

Clostridium perfringens, Enterotoxemia, Foals, PCR, ELISA

Volume

1

Article Issue

3

Related Issue

25087

Issue Date

2021-07-01

Receive Date

2021-03-15

Publish Date

2021-07-01

Page Start

1

Page End

8

Print ISSN

2735-4938

Online ISSN

2735-4946

Link

https://ejah.journals.ekb.eg/article_184692.html

Detail API

https://ejah.journals.ekb.eg/service?article_code=184692

Order

1

Type

Original researches

Type Code

1,636

Publication Type

Journal

Publication Title

Egyptian Journal of Animal Health

Publication Link

https://ejah.journals.ekb.eg/

MainTitle

Use of ELISA and polymerase chain reaction as rapid different methods in diagnosis of enterotoxemia in foals

Details

Type

Article

Created At

23 Jan 2023