Out of 450 samples collected from infertile egg ,dead in shell embryo ,baby chicks with omphalitis and hatcheries environment P. aeruginosa was identified in 12. 22% (55/450) of the total examined samples. Two strains (4%) were isolated from infertile egg,18 strains (12%) from dead in shell embryo ,33 strain(15%) from baby chicks and 2 strains (6.7%%) from hatcheries environment. Extended spectrum beta lactam( ESBL) production was confirmed in 8 isolates(14.55%) by double disk diffusion test . blaSHV and blaTEM genes were detected in (8/8)and (7/8) of ESBL-P. aeruginosa isolates respectively, whereas none of these isolates harbored, blaOXA and blaCTX-M genes. Molecular detection of (oprL) and (toxA) genes were amplified in in all eight studied strains. The results of antibiotic sensitivity for ESBL producing P. aeruginosa isolates indicated that the isolates were susceptible to norfloxacin, ciprofloxacin and colistin sulphate .On the other hand, all isolates were completely resistant to ampicillin, chloramphenicol, and trimethoprim-sulfamethoxazol and most of the isolates were resistant to streptomycin and tetracycline. The pathogenicity test of the isolated field strain of ESBL-P. aeruginosa proved to be highly pathogenic induced mortality rate of 43.33% . The predominant lesions of dead and sacrificed chicks were septicemia, congestion of internal organs (liver, heart& lung) and inflamed intestine. Histopathological findings supported the previous gross lesions. In conclusion, it can be said that there is an upsurge of beta lactamase producing Pseudomonas aeruginosa infer that one day old chicks and hatcheries can serves as a reservoir for growth and dissemination of clinically significant antibiotic resistance among bacterial species.