Lumpy skin disease (LSD) causes serious economic losses due to severe reduction in milk production, feed intake, and weight conversion, abortion, infertility, damage to cattle hide, suppressing immunity and deaths. LSD is considered a notifiable disease; Lumpy skin disease virus (LSDV) can be isolated from skin nodule collected from clinically infected cattle on chorioallantoic membrane (CAM) of embryonated chicken eggs (ECES) and identified by polymerase chain reaction (PCR). The present study describes the clinical and molecular diagnosis of lumpy skin disease (LSD) in Egypt during 2017. Twenty-one cases of cattle suspected of being infected with (LSDV) depending on the clinical signs which appear clearly. All suspected animals were clinically examined and a tentative diagnosis of LSD was done. Specimens (skin nodules) were collected from infected animals for isolation on Specific pathogen free (SPF) emberionated chicken eggs (ECE) chorio-alantoic membrane (CAMs) then examined by PCR assay to confirm existence of the disease causing agent in the suspected cases. The result of clinical examination revealed typical clinical manifestation of LSD with specific 570bp band that represent ORF103 gene of capripox virus was detected from selected sample by PCR.. Phylogeny was prouseeded using sequence of the partial ORF103 gene and by homology comparison with reference Lumpy skin viruses, SPVs and GPVs obtained from Gene Bank. The results showed 100% identity to LSDV/Menofiya1/18, LSDV/Menofiya 2/18, 99% LSDV/Kubash/KAZ/16, LSDV. Evros, 97% homology with goat pox virus GTPV. Sambalpur. KX398512.1 and 97% homology with sheep pox virus SPV/EG/AHRI/Wady El-gidid/MK 256477.1, SPV- El Minufiya - MF443334.1).