The kinetic and tissue distribution of cefquinome were studied after its intramuscular administration at a dose of 2 mg/kg b.wt in healthy and experimentally infected rabbits with klebsiella pneumoniae. The cefquinome serum concentration can be described by two-compartment model following single IM injection. The pharmacokinetic revealed that the values of distribution half-life (T_1/2alfa), maximum serum concentration (C_max), area under the curve (AUC_0–∞) and mean resident time (MRT) were significantly lower in infected rabbits than in healthy rabbits. Volume of distribution (V/F) and Total body clearance of drug (CL/F) was significantly higher in infected than in healthy rabbits. Repeated IM administration of cefquinome revealed that liver retains the highest drug concentrations in both healthy and infected rabbits while the lowest drug concentrations were found in muscle. The recommended withdrawal time is 7 for healthy and 3 days for K. pneumoniae infected rabbits to be safe for human consumption. Finally; K. pneumoniae infection caused significant alterations in some pharmacokinetic parameters and tissue concentrations of cefquinome in rabbits.