The current study deals with the prevalence of Mycoplasma mixed with Escherichia coli, Klebsiella sp. and Pseudomonas sp. causing respiratory diseases in sheep from different farms at Sharkia Governorate. A total of 110 different samples including; 30 nasal swabs and 80 lungs and tracheas were aseptically collected. The prevalence rates differ from one bacterial agent to another. M. ovipneumoniae was detected by31.8%, E. coli by 21.8%, Klebsiella sp. by 11.8% and finally, P. aeuroginosa by 9.1% from the collected samples. Biochemical and serological identifications revealed the presence of M. ovipneumoniae, E. coli of different serotypes, K. pneumoniae, K.oxytoca and P. aeuroginosa. Molecular characterization using PCR test targeting the 16SrRNA genes of these bacterial agents revealing their specific DNA amplicon sizes of each bacterium except for Klebsiella sp. which was targeted by gyrA gene. Various pictures of tracheal lesions in infected sheep were observed and recorded. Different types of pneumonia were observed macroscopically and microscopically among the different causitive bacterium. Antibiotic profiles show variety of sensitivity. Ciprofloxacin is the drug of choice for most isolates.P. aeuroginosa shows high level of antibiotics resistance.