Benzo(a) pyrene the first form of PAHs is genotoxic carcinogenic. This study was conducted to examine theBenzo(a) pyrene the first form of PAHs is genotoxic carcinogenic. This study was conducted to examine the potential therapeutic effects of levels of the extract of ashwagandha against testicular toxicity induced by BaP in rats. 30 mature rats were divided into 5 groups, (-) control group, BaP (10 mg/kg body weight), as (+) control BaP + treated with vit. E oral at a dose of 200 mg/kg body weight/day, BaP + AHE curative (1 ml /kg/day) and BaP + AHE curative (2 ml/kg /day) groups for 6 weeks. Many side effects were observed in animals injected with BaP such as loss of body weight, weakness activity and yellowish body hair. The results recorded a significant increase in body and testes weights, sperm count, sperm motility, serum levels of testosterone, luteinizing (LH), Follicle stimulating hormone (FSH), total antioxidants capacity (TAC), and (SOD) in the control-positive groups. And the groups curative with vitamin E and the levels of ashwagandha extract when compared to the negative control group, while a significant increase serum level MDA and luteinizing (LH) and in the BaP curative groups with vitamin E and the levels of ashwagandha extract compared to negative control group. Conclusions, administration at levels of ashwagandha extract caused ameliorative effect against BaP -induced testicular toxicity considered a powerful plant with health-promoting properties.