Abstract The present study investigated the effect of some drugs (Orlistat and Chitosan) and herbs (Neopuntia and Jamu Tea)  on weight, lipid fractions, glucose, liver function andkidney functions of obese rats fed on high fat diet. Healthy  male  albino  rats weighing (200±5 g) were divided into two main groups. The first group (n = 6) fed on a basal diet B.D., while the second main group 30 rat were fed for 6 weeks on high fat diet to induce the obesity in rats. After these period, rats were divided into 5 subgroups (n =6). As a following: Subgroup (1) fed on high fat and high protein diet  (20% fat and 20% protein) as a positive control. Subgroup (2) fed on high fat and high protein diet  and treated daily with 5 mg orlistat/ rat. Subgroup (3) fed on high fat and high protein diet  and treated daily with 5 mg chitosan/ rat. Subgroup (4) fed on high fat and high protein diet   and treated daily with 5 mg Neopuntia/ rat. Subgroup (5) fed on high fat and high protein diet  and treated daily with 5 ml Jamu tea/ rat.             Body weight was decreased in all treated groups. All parameters of lipid profile (cholesterol, triglycerides, HDL-c, LDL-c and VLDL-c), liver function (AST & ALT enzymes), glucose and kidney functions improved with treated the obese rats with Orlistat , Chitosan , Neopuntia and Jamu Tea, especially when using 5 mg orlistat/ rat followed by 5 mg chitosan / rat, and other herbs (Neopuntia and Jamu Tea), respectively . It was concluded that, treating  obese rats which fed on high fat and high protein diet with  Orlistat , Chitosan , Neopuntia and Jamu Tea decreased the weight of obese rats and improved the body functions. Key words: rats, obese,Orlistat , Chitosan , Neopuntia , Jamu Tea,  lipid profile, liver functions, glucose and kidney function.