Abstract Objective: The main target of this study was to investigate the effect of diets supplemented with three levels from Nigella sativa seeds (2.5, 5 and 10%) or water extract of Nigella sativa seeds in drinking water (2.5%, 5% and 10% W/V) on serum glucose, lipid profile, kidney functions and liver enzymes in addition to nutrition evaluation of rats suffering from hyperlipidemia and diabetes. Material & Methods: The experiment was carried out using 48 male albino rats (Sprague Dawley strain) weighting 130 ± 5 g. The rats were divided into two main groups. The first main group (n = 6) fed on basal diet as negative control. The second main group (n = 42) fed on hyperlipidimic diet for 6 week to induce hyperlipidemia, after this period, the second main group injected with (150 mg aloxane / kg body weight) to induce hyperglycemia. The rats in the second main group were randomly divided into seven subgroups as a following: Subgroup (1) fed on hyperlipidemic diet as a positive control group. Subgroups (2, 3 and 4) fed on hyperlipidemic diet containing 2.5%, 5% and 10% Nigella sativa seeds, respectively.  Subgroups (5, 6 and 7) fed on hyperlipidemic diet and treated daily with extract of Nigella sativa seeds in drinking water (2.5%, 5% and 10% W/V), respectively. All groups were kept feeding on experimental diets for 28 days. Results: The results indicated that, hyperlipidemia and hyperglycemia increased serum glucose, kidney functions, liver enzymes and lipid profile, significantly, except HDL-c., as compared to healthy rats. Treating rats which were suffering from hyperlipidemia and hyperglycemia with different levels from Nigella sativa seeds or water extract prepared from different levels from Nigella sativa led to a general improvement in health of rats, especially when used high level from seeds or water extract. Conclusion: It was finally concluded that, treated rats which suffer from hyperlipidemia and hyperglycemia with Nigella sativa seeds or water extract prepared from Nigella sativa seeds, especially when used high levels from seeds or extraction, could be implemented in the treatment of hyperlipidemic subjects suffering from diabetes. Key words: Hyperlipidemia, diabetes, rats, Nigella sativa, glucose, lipid profiles, kidney functions and liver enzymes.