Abstract This work was conducted to prepare kombucha tea from both black and white tea to study the possible use of kombucha tea to fight obesity using Sprague dawley male albeno rats. In the study the oral administration of the two kinds of kombucha tea for obese rats carried out to investigate the possible enhancement of slimming, by aid of kombucha tea with two herbal formulations which contained (1) Cornsilk + Juniper + Hawthorn + Thyme; (2) Alfalfa + Dandelion + Horstail + Bearberry. High fat diet used to induce obesity, and continued during 49 days of the feeding trail. Body wieght gain % (BWG %), feed intake (FI) and feed effeiciency ratio and some internal organs weights calculated as indication of biological changes. The biochemical parameters were determined in serum to find out the possible changes of liver functions, kidneys functions, lipids profile, creatinine, urea, uric acid and glucose. Nutritional intervention using kombucha white tea plus herbal formulation (2) revealed maxmum slimming with maintaining better the liver and kedneys function, the lipids profile with lowering serum glucose. Histopatiological changes confirmed that of biochemical parameters.