Abstract The aim of this work was to investigate the effect of biscuits fortified with pumpkin (Cucurbita) seeds with 15% of powder and with 3% of oil on amitriptyline induced liver in experimental rats. Twenty male rats of Sprague Dawley strain (weighting 190±10 g) were classified in to four groups ( 5 rats each). The first group fed on standard diet, used as a negative control group (-ve). Fifteen rats were given amitriptyline (10 mg/Kg) as aqueous solutions that prepared extempore and administered in traperitoneally (i.p) to rats once daily for 14 days . They divided into three groups, positive control group (+ve) the other two groups treated 15% pumpkin seeds powder biscuits and 3% pumpkin seeds oil biscuits. for 8 weeks. Rats received amitriptyline only without any treatment showed remarkable increases in serum levels lipid proifil indicating the outset of fatty liver. Greater raises inactivates of AST and ALT levels in serum were observed in positive control rats compared with those in normal control group. Treatment with 15% pumpkin seeds powder biscuits and 3% pumpkin seeds oil biscuits decreased the levels of serum cholesterol (TC), triglycerides (TG), high density lipoprotein (HDL-c), low density lipoprotein (LDL-c) and very low density lipoprotein (VLDL-c) in serum linked with reduction in the activity of serum AST and ALT. Also the results observed in groups treatment with 15% pumpkin seeds powder biscuits and 3% pumpkin seeds oil biscuits showed remarkable increases in total antioxidants , Superoxide dismutase (SOD) , weight gain , food intake , food efficiency and HDL-c. Also decreased the levels of nitric oxide (NO) compared with positive control rats .These results suggest that biscuits fortified with both of pumpkin seeds powder and its oil can be used as health food with functional properties for treatment of fatty liver induced by amitriptyline. Key of words: Fatty liver - Lipid profile - Amitriptyline- Pumpkin seeds - Total antioxidants.