Background: Positive Organizational learning culture can help the staff nurses to improve their existing skills and knowledge and provide opportunities for them to discover better ways of working together in order to become self-directed, independent, and active learner, evidence of staff nurses achievement goals can provide guidelines on how learning settings need to modification so that learning can be adjusted. Aim: This study aimed to assess the relation between organizational learning culture and goal orientation among staff nurses. Design: Descriptive correlational research design was used. Setting: The study conducted in the Medical and Surgical departments at Benha University Hospital. Subjects:  Convenient sample of all available staff nurses who are working in the above-mentioned study setting (n=322). Tools of data collection: Two tools were used; Organizational Learning Culture Questionnaire, and Goal Orientation Questionnaire. Results: Showed that the (68.0%) of staff nurses reported that organizational learning culture was high, and (79.9%) of them reported that goal orientation level was moderate. Conclusion: There was a highly positive statistically significant relation between total organizational learning culture level and total goal orientation level. Recommendation: Nursing administrator need to continually look for opportunities to learn and support staff nurses' requests to participate in training courses, collaboration should be maintained between nursing managers and nurses through effective communication and education to effectively enhance organizational learning culture.