Background: Uterine prolapse occurs when the uterus descends towards or through the opening of the vagina below its normal anatomical position. Aim of study: Was to evaluate effect of nursing intervention package on prevention of uterine prolapse among pregnant women in third trimester. Design: A quasi experimental design was utilized to fulfill the aim of the study: two groups were studied (study and control group) with pre, post and follow up test. Setting: The study was conducted at obstetrics and gynecology outpatient clinic at Benha university hospital. Sample: A purposive sample of 90 women was selected according to the inclusion criteria. Tools of data collection: Four tools were included, a structured interviewing questionnaire sheet, women's reported practices sheet toward prevention of uterine prolapse, modified  Likert scale and  assessment tool for uterine displacement. Results: There were a highly statistical correlation between total knowledge, total practices, and total attitude among studied women (study and control groups)  as (p ≤0.000)  and (p ≤ 0.006) .Also women who applied the nursing intervention package (study group)  had no degrees  of uterine prolapse in  the follow up test. Conclusion: There was a highly statistical improvement in the study group than control group regarding the total level of knowledge, practice and attitude about prevention of uterine prolapse post- test. Recommendation: Develop awareness programs for the pregnant women to improve their knowledge, practice and attitude about uterine prolapse.