Background: Abnormalities of the genitourinary system account for the majority of urogenital disorders in children. External genitourinary malformations are easily identified at birth, while internal defects can be specific when symptoms develop in late infancy. Aim of study: Was to evaluate the effect of maternal preoperative guidelines for surgical recovery of urogenital abnormalities on the child's surgical outcome. Research design: Quasi-experimental design was used in the study. Setting: The study was conducted at Benha Specialized Hospital for Children affiliated to Ministry of Health and Population. Sample: 60 mothers with their children were included in the study. Tools of data collection: Included three tools , Tool (I) ; Structured Interviewing Questionnaire Sheet to assess personal characteristics of mothers and children, mother's knowledge regarding to care of their children undergoing surgical repair of genitourinary anomalies, Tool (II) : Assessment Sheet to assess mother's practical knowledge regarding to care of their children undergoing surgical repair of genitourinary anomalies, Results: There was highly significant improvement in the studied mothers' total level of knowledge (98.8% had satisfactory knowledge) and total practice post implementation. Conclusion: There is a highly significant positive correlation between mothers' total knowledge and their total practice scores regarding genito-urinary anomalies. Recommendations: Providing sufficient training for mothers' pre operative instructions regarding to surgical repair of genitourinary anomalies on their children postoperative outcomes to enhance their knowledge and practice.