Background: Six sigma is an approach of quality management, which is intended to improve the healthcare services, and processes by continuously reducing defects due to excessive process variation in health care. Aim of study: Was to assess nursing staff readiness regard utilization of six sigma of quality management. Design: A descriptive correlational design was used. Setting: The study was conducted in critical care unit at Banha University Hospital. Subjects: A Convenient sample consisted of 266 of nursing staff divided into (236 staff nurses and 30 head nurses). Tools: Two tools were used: 1- Six sigma knowledge questionnaire and 2-Six sigma readiness questionnaires. Results: The highest percent of nursing staff had fair knowledge level regard utilization of six sigma of quality management. Also, this result showed that the majority of nursing staff had low readiness level regarding utilization of six sigma of quality management. Conclusion: There were no statistically significant relation between nursing staff knowledge and their readiness toward using of six sigma of quality management. Recommendation: Hospital administration should provide training programs to increase nursing staff awareness of six sigma and their readiness to apply six sigma in their work place.