Background: Monitoring and recording fetal heart rate by cardiotocography is to ‎detect any changes that might occur that put the fetus at risk of fetal distress or ‎death due to lack oxygen. Aim of study: Was to evaluate the effect of educational program ‎on knowledge and practices of maternity nurses regarding cardiotocography. ‎Design: A quasi-experimental design (Time series design) was utilized to fulfil the ‎aim of the study. Sample: A convenient sample of 40 maternity nurses were ‎included in the present study. Setting: The present study was conducted at Labor ‎and Cardiotocography room of the Obstetrics and Gynecological department at Benha ‎University Hospital. Tools of data collection: Self-administered ‎questionnaire, and nurses' observational checklist regarding cardiotocography. ‎Results: The majority of nurses were had poor level of knowledge and ‎unsatisfactory practice during pre-program, while during post program, and follow-‎up the majority of nurses were had good level of knowledge and satisfactory ‎practice, with slightly decline during after 3 months follow-up. Conclusion: Educational program ‎had a positive effect on improving knowledge and practice of maternity nurses ‎regarding cardiotocography. Recommendations: Applying of continuous in-service ‎training program for maternity nurses to improve performance regarding cardiotocography.