Background: Umbilical cord blood banking is a surviving procedure that can increase life expectancy of end-stage or chronically ill patients. Aim of study: Was to evaluate the effect of an educational program on maternity nurses' knowledge and attitude about umbilical cord blood banking and its barriers. Design: A quasi-experimental pre-posttest research design was utilized. Setting: The study was conducted at Obstetrics and Gynecological departments at Benha University Hospital. Sampling: A convenient sample of 100 maternity nurses. Tools of data collection: Two main tools were used, I: A structure interviewing questionnaire which included 2 parts I) socio-demographic characteristics of maternity nurses and II) knowledge of maternity nurses regarding umbilical cord blood banking and its barriers and II: Modified Likert scale was adopted to assess the nurses' attitudes regarding to umbilical cord blood banking and its barriers. Results: There was a highly statistical significant difference between the results of immediate post program compared to pre program in favor of post program. Additionally, there were positive highly statistical significant correlations between total knowledge score and total attitude score pre and immediate post program. Conclusion: There was significant improving in maternity nurses' knowledge and attitude regarding umbilical cord blood banking and its barriers. Therefore, the research hypothesis was supported and the aim was achieved. Recommendations:  Continuously planned in-service training programs related to cord blood banking must be established to develop nurses' knowledge, attitude in order to suit newly developed concepts in care.