Background: COVID-19 affects on nurses psychological attitudes.  Nurses often face, unfamiliar environment in the isolation unites, and workload, long hours, these may increase the level of anxiety, depression and stress among nurses. Aim of study: Was toassess the effect of covid-19 on psychological status among nurses working in isolation hospital. Research design: A descriptive design was utilized. Sample:  A convenience sample of 169 nurses included in the study. Setting: The study was conducted at Qaha isolation hospital in Qalyubiyah Governorate Tools of data collection: Two tools were used. I: An interviewing questionnaire to assess nursing staff socio demographic data, occupational factors and experience acquired to nurses from caring of covid-19 patients.  II: Depression, Anxiety and Stress Scale (DASS- 21). Results: Slightly less than half of the studied nurses had moderate level of depression while slightly more than one third of them had severe anxiety feeling and slightly less than half of the studied nurses had moderate feeling of stress. There was highly statistically significant between total level of depression and total level of stress among studied nurses. In addition, that there is positive correlation between total score of depression, anxiety and total score of stress among studied subjects. Conclusion: The overall psychological status of studied nurses was generally poor during COVID-19 outbreak, and several impact factors associated with nurses' psychological health were identified. Recommendations: Paying attention to nurses' psychological health status while fulfilling their responsibilities especially during sudden disaster as COVID-19.