Background: Preventive strategies are more effective in preventing klebsiella infection which stands out as the main microorganism resulting in infection between neonates in neonatal intensive care unit. Aim of the study: Was to evaluate the effect of preventive strategies for nurses regarding klebsiella infection in neonatal intensive care unit. Design: A quasi-experimental research design was used to carry out the study. Setting: This study was carried out in Neonatal intensive care unit at Benha University Hospital. Sample: A convenience sample of (90) nurses & a purposive sample of (90) neonates. Tools of data collection: Tool (I): A structured interviewing questionnaire sheet. Tool (II): Nursing observational checklist which was adopted from. Tool (III): Nurses' attitude rating scale regarding preventive strategies of klebsiella infection, and Tool (IV): Blood culture and C - reactive protein (CRP). Results: The majority of the studied nurses had good knowledge, competent practice and positive attitude about preventive strategies regarding klebsiella infection post-preventive strategies implementation compared with pre-preventive strategies implementation. Also, there was statistical significant difference among the studied neonates regarding klebsiella infection pre & post-preventive strategies implementation. Conclusion: Improved nurses' knowledge, practice and attitude regarding preventive strategies of klebsiella infection positively affected klebsiella infection rate among neonates. Recommendations: Emphasis on the importance of the preventive strategies regarding klebsiella infection for neonates of the study setting.