Background: Improving nursing knowledge and practice regarding safety measures is very essential that can achieve through developing standards of cardiac catheter nursing intervention, clear identification of deficiencies in provision of care and increasing nurses' awareness regarding their vital role in saving patient life through safe practice Aim: This study aimed to evaluate nurses' performance regarding safety measures in cardiac catheterization unit. Design: Descriptive research design was utilized. Setting: This study was conducted in cardiac catheterization unit, at Benha University Hospital. Sample: A convenience sample of 40 cardiac nurses from both sex were involved in the current study. Tools: Three tools were used, I: Structured interviewing questionnaire to assess nurses' knowledge regarding safety measures, II: Safety measuresobservational checklist to assess nurses' practice and III: Safety attitude questionnaire sheet to assess nurses' attitude. Results: 60% of studied nurses had unsatisfactory level of total knowledge  about safety measures and 75% of studied nurses had incompetence level of  total practice about safety measures in cardiac catheterization unit, while 77.5% of the studied nurses had positive attitude towards safety culture in cardiac catheterization unit. Conclusion: The nurses' performance was unsatisfactory regarding to safety measures. There was highly statistically significant relation between total nurses' knowledge, practice and attitude of studied nurses and demographic characteristics such as educational level , years of experience and attendance of training courses at  (P= < 0.01). Recommendations: Future study should be conducted to evaluate effect of an educational program regarding safety measures on nurses' performance.