Background: Breast Cancer is the most common malignancy and the second leading cause of cancer deaths among women. It is the most common invasive cancer as it affects about 12% of women worldwide. The study aimed to evaluate the effect of the program on improving women knowledge and practice regarding breast cancer and self-examination. Research design: A quasi-experimental research design was utilized in this study. Setting: The study was conducted at Benha University. Sample: A simple random sample included 375women who were worked at Benha University. Tools: two tools were used. I: A structured interviewing questionnaire which consisted of two parts. II: An observation checklist to assess studied women` practices regarding breast self- examination. Results: There was highly statistically significant difference among all items of studied women knowledge about breast cancer pre and post implementation of educational program. There was highly statistically difference among all items of studied women practices about exercises, physical activities and smoking pre and post implementation of educational program. Conclusion: There was statistically significant relation between the studied women total knowledge and their total practices pre and post implementation of educational program p> 0.05. Recommendations: Continuous health educational program for women regarding breast cancer  in Egypt building on the president's initiative to support women's health for early detection of breast cancerand future research is proposed to explore and generate the effect of health educational program to enhance breast self-examination among women.