Acetamiprid (ACMP) is a widely used neonicotinoid that is frequently favored in pest control. After imidacloprid, it is the most commonly manufactured neonicotinoid. It was established that ACMP exhibits brain toxic impact at sub-lethal doses. Curcumin (Cur) a natural polyphenol has been conveyed to possess neuroprotective activity by reducing oxidative stress. The purpose of this study was to look into the neuroprotective role of curcumin against acetamiprid induced neuro-toxicity. In the current study, seventy adult male albino rats were divided into two main groups 20 days and 40 days each of them subdivided into 5 groups consist of: control, vehicle (DMSO), Cur, ACMP and ACMP +Cur group. The current study found that acetamiprid administration resulted in a significant increase in nitric oxide levels (NO) with histopathological changes in brain hippocampus tissue. Curcumin co-administration with acetamiprid significantly reduced nitric oxide levels and protected against acetamiprid-mediated structural changes in the hippocampal tissue.