The present work investigated the addition effect of vitamins (E + C) to two types of zinc oxide bulk and nano (ZnOBPs,ZnONPs) on cholesterol and albumin levels in Oreochrmis niloticus. The studied fish groups were subjected to 1/8, 1/4 and 1/2 sublethal concentrations (LC50) under different exposure period. The results of serum albumin give a significant decreasing (P0.05) when compared with the control group. On the other side, when under study fish groups were subjected to the same concentration of ZnO (bulk or nano) supplemented with vitamin (E and C) a significant increasing (P0.05) in serum albumin level were observed. The maximum value of fish exposed to ZnOBPs and ZnONPs plus vitamin (E and C) was (2.77±0.09 and 2.68±0.10 g/dl respectively) recorded at 1/8 LC50 concentration after 7 day .In case of cholesterol concentration, fish groups subjected to sublethal concentrations of ZnO bulk or nano, showed significant increased (p≤0.05). However, the addition of vitamins (E and C) lead to a significant decreasing (P0.05) in cholesterol concentration when compared to the group that have no vitamins.