In the current paper, the effect of presence and absence of synchrotron on quantitative analysis of sample is investigated using Fourier transform filters method. Using Raman spectroscopy on cancer tissues sample, which is one of the most important herbs, quantitative and qualitative analyses are performed. DNA/RNA of cancer cells was detected in the sample and the performance of Raman arrangement for measuring DNA/RNA of cancer cells concentration was evaluated at two parts using calibration graph. In the first part, spectra are containing synchrotron while in the second part, spectra are filtered and synchrotron are removed.