Fipronil (FPN), a phenyl pyrazole insecticide, has gotten a lot of attention recently because of its toxicity in mammals, which is mostly mediated through its effects on the antioxidant system. Quercetin is a flavonoid present in many fruits and vegetables that has been demonstrated to have a variety of beneficial biological effects, including anti-inflammatory and antioxidant properties. Nanoparticles of quercetin have been shown to improve its solubility and stability, allowing for improved absorption, cellular absorption, and lower toxicity. The present study was undertaken to explore the prophylactic effect of quercetin nanoparticles (QueNPs) against sub-chronic FPN-induced hepatic injury and oxidative stress in male rats. Experimental animals were orally gavaged for four weeks. It has been shown that FPN elevated the sera of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) whereas serum total protein level was decreased. FIP also caused histological changes in hepatic tissue. The QueNPs counteracted the hepatotoxic effect of FPN exposure.