Escherichia coli O157:H7 is a major food borne pathogens of concern worldwide so, the present study was carried out for isolation of E. coli O157:H7, molecular characterization, detection of virulence genes, antibiogram and phenotypic detection of Extended spectrum beta lactamase (ESBL) producers among isolated strains from raw camel milk goat meat and minced goat meat. A total of 66 samples (44 raw camel milk, 15 goat meat and 7 minced goat meat) were collected from local retail outlets, butcher's shop and super markets in Matrouh Governorate. Samples were subjected to bacterial isolation and identification using cultural, biochemical, PCR, antibiogram and phenotypic detection of ESBL. Out of 66 samples, 7 (10.6%) E. coli O157:H7 isolates were recovered. All 7 (100%) tested isolates were positive for flicH7 gene. 4 (57.14%) isolates were positive for stx1 but, none of the isolates were positive for stx2 gene. E. coli O157:H7 isolates showed susceptibility to gentamycin (28.6%), chloramphenicol (57.1%), cefepime and cefuroxime (14.3%) while intermediate susceptible to nalidixic acid (71.4%), streptomycin (57.1%), chloramphenicol, ampicillin and cefepime (14.3%), but resistant to cefotaxime and ceftazidime (100%), ampicillin and cefuroxime (85.7%), gentamycin and cefepime (71.4%), streptomycin (42.9%), nalidixic acid and chloramphenicol (28.6%). only 14.3% of isolates were ESBL producers. Consequently, the goat meat is one of the major sources of E. coli O157:H7 in Matrouh Governorate. Multiple antibiotic resistant profiles of E. coli O157:H7 show a risk for public health and food safety as well as animal production so, proper antibiotics usage in animal production also needs attention.