The intensive and uninformed use of pesticides has become always a serious environmental issue worldwide. Synthetic and natural pesticides are expected to possess toxicities including genotoxicity. The present study was conducted to investigate the cytotoxicity and genotoxicity induced by commercial formulations of fluazifop-P-butyl (FPB) herbicide and acetamiprid (ACM) insecticide using Allium cepa assay. The result of root growth analysis indicated that FPB and ACM significantly inhibited the root growth by 70.5 and 51.6% at concentration of 0.1 ppm. The EC50 values determined after 96-h exposure were 0.024 and 0.072 ppm for FPB and ACM, respectively. A concentration-dependent inhibition in the total mitotic index (M In %) ranged from 44.4 to 84.7 % was observed at all evaluated concentrations of FPB, representing ⅟10, ⅟2 and EC50, compared to that of the control. The higher concentration (EC50) of ACM also induced significant inhibition in MI by 67.6 %. All concentrations of tested FPB induced severe damage during the mitotic division. Different forms of chromosome aberrations were also detected at all concentrations tested of ACM. The present data revealed the potential risk of both pesticides, at tested concentrations, inducing genotoxicity on non-target organism. Also, it is concluded that A. cepa test can be used as a sensitive indicator to detect possible genotoxicity of chemicals.