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277041

Immunomodulatory Properties of Human Adipose Mesenchymal Stromal/Stem Cell in Type 2 Diabetes Milieu.

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Last updated: 27 Dec 2024

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Abstract

Adipose tissue is a readily available and plentiful source of multipotent mesenchymal stromal/stem cells (AT-MSC). The immunomodulatory properties of AT-MSC are being introduced in type 2 diabetes (T2D) cell- based therapy. The study aimed to uncover the impact of T2D, on the interplay between AT-MSC and immune cells to develop an effective and safe AT-MSC immunotherapeutic modality. Thus, a direct allogenic co-culture of healthy AT-MSC (nAT-MSC) and peripheral blood mononuclear cells (PBMC), from healthy (nPBMC) or T2D (dPBMC) donors, and stimulated with anti-CD3/CD28, was established in vitro. PBMC proliferation was evaluated by measuring 5-bromo-20-deoxyuridine (Brdu) incorporation in the DNA of proliferating cells in a colorimetric ELISA assay. CD3+ T cell activation surface markers (CD25 and HLA-DR) expression was detected using a flow cytometer. As well, the anti-proliferative effect of naïve and interferon gamma (IFN-ɤ) - primed AT-MSC, isolated from T2D patients (dAT-MSC), on autologous PBMC was explored using the Brdu proliferation assay. In the applied co-culture setting, we observed that the diabetic milieu did not significantly impact the potential of nAT-MSC to suppress stimulated PBMC proliferation. However, it significantly compromised nAT-MSC ability to modulate the activation markers expression, making them less potent to suppress CD25 and HLA-DR expression. Moreover, the dAT-MSC had attenuated ability to suppress the proliferation of autologous stimulated dPBMC, nevertheless, priming of dAT-MSC with IFN-ɤ, could, to an extent, improve such defect. The results suggest that T2D might affect the immunosuppressive potential of AT-MSC and pre-conditioning of dAT-MSC with a pro-inflammatory stimuli could enhance their therapeutic effect.

DOI

10.21608/aijpms.2022.150124.1153

Keywords

Type 2 Diabetes, Adipose, mesenchymal, Anti-proliferative, PBMC

Authors

First Name

Marwa

Last Name

Elshahat

MiddleName

M

Affiliation

Stem Cell Research Group, Medical Research Centre of Excellence, National Research Centre, Cairo, Egypt. , Department of Medical Molecular Genetics, Human Genetics and Genome Research Division, National Research Centre, Cairo, Egypt

Email

marwa_aromatic_85@yahoo.com

City

-

Orcid

0000-0003-2954-4573

First Name

Nourhan

Last Name

Abu-Shahba

MiddleName

M

Affiliation

Stem Cell Research Group, Medical Research Centre of Excellence, National Research Centre, Cairo, Egypt. , Department of Medical Molecular Genetics, Human Genetics and Genome Research Division, National Research Centre, Cairo, Egypt

Email

nourajina@hotmail.com

City

-

Orcid

0000-0002-3736-4655

First Name

Ghada

Last Name

Nour El-Din

MiddleName

M

Affiliation

Stem Cell Research Group, Medical Research Centre of Excellence, National Research Centre, Cairo, Egypt, Department of Molecular Genetics and Enzymology, Human Genetics and Genome Research Division, National Research Centre, Cairo, Egypt

Email

genoghada@ymail.com

City

-

Orcid

-

First Name

Raghda

Last Name

Ghorab

MiddleName

M

Affiliation

Department of Immunogenetics, Human Genetics and Genome Research Division, National Research Centre, Cairo, Egypt

Email

raghdaghorab@hotmail.com

City

-

Orcid

-

First Name

Alaa

Last Name

El-Erian

MiddleName

M

Affiliation

Department of Endocrine Surgery, National Institute of Diabetes and Endocrinology, Cairo, Egypt

Email

alaerian_nasr@gothi.gov.eg

City

-

Orcid

-

First Name

Khalda

Last Name

Amr

MiddleName

S

Affiliation

Department of Medical Molecular Genetics, Human Genetics and Genome Research Division, National Research Centre, Cairo, Egypt

Email

khalda_nrc@yahoo.com

City

-

Orcid

0000-0001-8472-5911

First Name

Osama

Last Name

Azmy

MiddleName

M

Affiliation

Stem Cell Research Group, Medical Research Centre of Excellence, National Research Centre, Cairo, Egypt, Department of Reproductive Health Research, Medical Research Division, National Research Centre, Cairo, Egypt, Egypt Center for Research and Regenerative Medicine, Cairo, Egypt

Email

osamaazmy@yahoo.com

City

-

Orcid

0000-0003-0354-6597

First Name

Abeer

Last Name

Abd El-Fattah

MiddleName

Ibrahim

Affiliation

Department of Biochemistry and Molecular Biology, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt

Email

abeeribrahim75@yahoo.com

City

Cairo

Orcid

-

Volume

3

Article Issue

1

Related Issue

38444

Issue Date

2023-01-01

Receive Date

2022-07-20

Publish Date

2023-01-01

Page Start

144

Page End

155

Print ISSN

2735-4598

Online ISSN

2735-4601

Article File

AIJPMS_Volume 3_Issue 1_Pages 144-155.pdf

PDF . 790.9kB

Link

https://aijpms.journals.ekb.eg/article_277041.html

Detail API

https://aijpms.journals.ekb.eg/service?article_code=277041

Order

14

Type

Original research articles

Type Code

1,562

Publication Type

Journal

Publication Title

Azhar International Journal of Pharmaceutical and Medical Sciences

Publication Link

https://aijpms.journals.ekb.eg/

MainTitle

Immunomodulatory Properties of Human Adipose Mesenchymal Stromal/Stem Cell in Type 2 Diabetes Milieu.

Details

Type

Article

Created At

23 Jan 2023