The objective of this study is to formulate and evaluate a topical gel containing Ectoine for further use in treating skin diseases. Three gel formulations were prepared using 1.5%, 2% and 3% of gelling agent HPMC K15M and these formulae were evaluated for their physical appearance, pH, spreadability, viscosity, in vitro release profile, freeze thaw cycle and ex vivo release. The preformulation studies for both Ectoine and HPMC were applied using DSC and FTIR. HPLC analysis of the natural compound was conducted to detected concentration of Ectoine in formulated gels. Formulated gels were homogenous, had good consistency, spreadability but unstable when subjected to high temperature. Among the three formulae, H1 showed better release (91.4%) characteristics than other concentrations. The kinetic data of the release investigated that Ectoine release from all examined gel followed the diffusion mechanism except H1 was first order. The selected formula showed good release through rat skin (85%) after 8hrs.