Acute ischemic stroke is one of the diseases causing death worldwide. MicroRNAs have pivotal roles in acute ischemic stroke pathogenesis, and their expression dysregulation, sensitivity and circulating stability could promote them as diagnostic biomarkers. So, this study aimed to examine the expression fold change and diagnostic accuracy potential of miR-601 and miR-760. The expression pattern of miR-601 and miR-760 in the serum of the 70 acute ischemic stroke patients in comparison to 25 age-matched control subjects was estimated using quantitative real time polymerase chain reaction. The receiver operator curve analysis was performed to determine the diagnostic potential of the studied microRNAs. Down-expression of miR-601 and miR-760 was found (94.8%; -1.16 ±1.05; 45; 75.8%; -0.525±0.936, respectively), the diagnostic accuracy was higher for miR-601 (AUC = 0.859), while the there was no diagnostic accuracy for miR-760. On comparing the studied miRNAs, miR-601 showed the best accuracy than miR-760 regarding the diagnosis of stroke.