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208551

Amelioration of Bile Duct Ligation Induced Liver Injury by Lactoferrin: Role of Nrf2/HO-1 Pathway

Article

Last updated: 27 Dec 2024

Subjects

-

Tags

Pharmacology and toxicology

Abstract

Cholestasis is one of the major determinants which causes hepatic injury and might lead to liver complications. In cholestasis, toxic bile acids accumulate with decrease in the capacity of detoxification. It can be stimulated experimentally by bile duct ligation (BDL) to block the bile flow from the liver. As for the treatment, lactoferrin (LF), the iron-binding glycoprotein, has been evaluated in various studies for the treatment of infections, inflammation, and cancer. This study aims to illustrate the curative effects of LF on BDL-induced hepatic injury in rats. Animals were randomly allocated into 4 groups: the first group is the control sham; the subjects of the second group have been subjected to a surgery of BDL; the latter procedure has been induced in the third group, 14 days later, they have been subjected to the treatment with LF (300 mg/kg/day, po) for two weeks; as for the last group, LF has been administered (300mg/kg/day, po) for two weeks. BDL elevated biomarkers of hepatocellular damage (aspartate aminotransferase (AST) and alanine aminotransferase (ALT)) and obstructive cholestatic injury (gamma-glutamyl transferase (γ-GT) and total bilirubin). In addition, it provoked oxidative stress manifested by increase in hepatic MDA and reduction of SOD with down-regulation of Nrf2/HO-1 pathway. LF treatment ameliorated these effects through its antioxidant activity by activation of Nrf2/HO-1 pathway that leads to decrease in hepatic MDA and elevation of SOD. In conclusion; lactoferrin alleviated hepatic injury induced by BDL via activating Nrf2 gene expression that might be led back to its antioxidant properties.

DOI

10.21608/aijpms.2021.78232.1076

Keywords

Cholestasis, Lactoferrin, Bile Duct Ligation (BDL), Nrf2, Oxidative Stress

Authors

First Name

Aya

Last Name

Ayob

MiddleName

Reda

Affiliation

Department of Pharmacology and Toxicology, Faculty of Pharmacy, 6th of October University, Giza, Egypt.

Email

dr.ayaayob@yahoo.com

City

cairo

Orcid

-

First Name

Aya

Last Name

Al-Najjar

MiddleName

-

Affiliation

Pharmacology and toxicology Department. Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt

Email

phayahesham@gmail.com

City

-

Orcid

-

First Name

Azza

Last Name

Awad

MiddleName

-

Affiliation

Pharmacology and toxicology Department. Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt

Email

azza_mohie@hotmail.com

City

cairo

Orcid

-

Volume

1

Article Issue

3

Related Issue

29111

Issue Date

2021-11-01

Receive Date

2021-05-30

Publish Date

2021-11-01

Page Start

84

Page End

90

Print ISSN

2735-4598

Online ISSN

2735-4601

Link

https://aijpms.journals.ekb.eg/article_208551.html

Detail API

https://aijpms.journals.ekb.eg/service?article_code=208551

Order

8

Type

Original research articles

Type Code

1,562

Publication Type

Journal

Publication Title

Azhar International Journal of Pharmaceutical and Medical Sciences

Publication Link

https://aijpms.journals.ekb.eg/

MainTitle

Amelioration of Bile Duct Ligation Induced Liver Injury by Lactoferrin: Role of Nrf2/HO-1 Pathway

Details

Type

Article

Created At

23 Jan 2023